Anti-Prosurfactant Protein B/SFTPB Antibody Picoband®

About this product

Boster Bio Anti-Prosurfactant Protein B/SFTPB Antibody Picoband® catalog # A03441-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03441-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Pulmonary surfactant-associated protein B is a protein that in humans is encoded by the SFTPB gene. This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.

Synonyms

Pulmonary surfactant-associated protein B; SP-B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL (Phe) ; SFTPB; SFTP3

Gene Name

SFTPB

Gene ID

6439

UniProt

P07988

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SFTPB, which shares 80% amino acid (aa) sequence identity with both mouse and rat SFTPB.

Clonality

Polyclonal

Tissue Specificity

Found in the synovial fluid of patients with rheumatoid arthritis. .

Applications

WB

Field of Research

Cancer, Cardiovascular, Cell Biology, Cell Cycle, Cell Differentiation, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Vasculature

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

References & Citations

1. Tredano, M., Cooper, D. N., Stuhrmann, M., Christodoulou, J., Chuzhanova, N. A., Roudot-Thoraval, F., Boelle, P.-Y., Elion, J., Jeanpierre, M., Feingold, J., Couderc, R., Bahuau, M. Origin of the prevalent SFTPB Indel g.1549C-GAA (121ins2) mutation causing surfactant protein B (SP-B) deficiency. Am. J. Med. Genet. 140A: 62-69, 2006. 2. Tredano, M., van Elburg, R. M., Kaspers, A. G., Zimmermann, L. J., Houdayer, C., Aymard, P., Hull, W. M., Whitsett, J. A., Elion, J., Griese, M., Bahuau, M. Compound SFTPB 1549C-to-GAA (121ins2) and 457delC heterozygosity in severe congenital lung disease and surfactant protein B (SP-B) deficiency. Hum. Mutat. 14: 502-509, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

31203 MW

Observed Molecular Weight

46 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Surfactant protein B

Subcellular Location

Secreted, extracellular space, surface film.

Protein Name

Tumor necrosis factor-inducible gene 6 protein

Isotype

Rabbit IgG

Contents

243