Anti-TRPC4 Antibody Picoband®

About this product

Boster Bio Anti-TRPC4 Antibody Picoband® catalog # A02037-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02037-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The short transient receptor potential channel 4 (TRPC4), also known as Trp-related protein 4, is a protein that in humans is encoded by the TRPC4 gene. TRPC4 is a member of the transient receptor potential cation channels. It is mapped to 13q13.3. This protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors andtyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. TRPC4 also has an essential role in endothelial-dependent regulation of vascular tone, endothelial permeability, and neurotransmitter release from thalamic interneurons.

Synonyms

Short transient receptor potential channel 4; TrpC4; Trp-related protein 4; hTrp-4; hTrp4; TRPC4

Gene Name

TRPC4

Gene ID

7223

UniProt

Q9UBN4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human TRPC4, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Strongly expressed in placenta. Expressed at lower levels in heart, pancreas, kidney and brain. Expressed in endothelial cells. Isoform alpha was found to be the predominant isoform. Isoform beta was not found in pancreas and brain.

Applications

WB

Field of Research

Calcium Channels, Calcium Signaling, G Protein Signaling, Neuroscience, Neurotransmission, Protein Phosphorylation, Receptor Tyrosine Kinases, Receptors / Channels, Signal Transduction, Signaling Pathway, Tyrosine Kinases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Form a receptor-activated non-selective calcium permeant cation channel. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Mediates cation entry, with an enhanced permeability to barium over calcium. May also be activated by intracellular calcium store depletion.

References & Citations

1. Entrez Gene: transient receptor potential cation channel, subfamily C, member 4 2. Freichel, M., Vennekens, R., Olausson, J., Hoffmann, M., Muller, C., Stolz, S., Scheunemann, J., Weissgerber, P., Flockerzi, V. Functional role of TRPC proteins in vivo: lessons from TRPC-deficient mouse models. Biochem. Biophys. Res. Commun. 322: 1352-1358, 2004. 3. Zhu X, Jiang M, Peyton M, Boulay G, Hurst R, Stefani E, Birnbaumer L (May 1996) . trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry. Cell 85 (5) : 661–71.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

37492 MW

Observed Molecular Weight

150 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Transient receptor potential cation channel, subfamily C, member 4

Subcellular Location

Membrane; Enhanced insertion into the cell membrane after activation of the EGF receptor.

Protein Name

Tripartite motif-containing protein 6

Isotype

Rabbit IgG

Contents

243