Anti-Bikunin/AMBP Antibody Picoband®

About this product

Boster Bio Anti-Bikunin/AMBP Antibody Picoband® catalog # A02419-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02419-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. Human bikunin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa A1M protein (aa 20-203), and the C-terminal serine protease inhibitor bikunin (aa 206-352) .

Synonyms

Protein AMBP; Alpha-1-microglobulin; Protein HC; Alpha-1 microglycoprotein; Complex-forming glycoprotein heterogeneous in charge; Inter-alpha-trypsin inhibitor light chain; ITI-LC; Bikunin; EDC1; HI-30; Uronic-acid-rich protein; Trypstatin; AMBP; HCP; ITIL

Gene Name

AMBP

Gene ID

259

UniProt

P02760

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Bikunin/AMBP recombinant protein (Position: A206-N352) .

Clonality

Polyclonal

Tissue Specificity

Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha- trypsin inhibitor is present in plasma and urine.

Applications

ELISA, WB

Field of Research

Cancer, Cardiovascular, Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin, Serum Proteins, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

References & Citations

1. Entrez Gene: AMBP alpha-1-microglobulin/bikunin precursor. 2. Salier JP, Simon D, Rouet P, Raguenez G, Muscatelli F, Gebhard W, Guenet JL, Mattei MG (Dec 1992) . Homologous chromosomal locations of the four genes for inter-alpha-inhibitor and pre-alpha-inhibitor family in human and mouse: assignment of the ancestral gene for the lipocalin superfamily. Genomics. 14 (1) : 83–8. 3. Vetr H, Gebhard W (Jun 1991) . Structure of the human alpha 1-microglobulin-bikunin gene. Biol. Chem. Hoppe-Seyler. 371 (12) : 1185–96.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

34 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Inter-alpha-trypsin inhibitor light chain

Subcellular Location

Secreted.

Isotype

Rabbit IgG

Contents

243