Anti-Caspase 4/Casp4 Antibody Picoband®

About this product

Boster Bio Anti-Caspase 4/Casp4 Antibody Picoband® catalog # A02941-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02941-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. The Caspase 4 gene is mapped to a P1 clone containing the ICE gene, which is located at chromosome 11q22.2-q22.3. It contains 8 coding exons. The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5 (and the murine homolog caspase 11), with a role in the immune system.

Synonyms

Caspase-4; CASP-4

Gene Name

Casp4

Gene ID

12363

UniProt

P70343

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived mouse Caspase 4 recombinant protein (Position: E108-T195) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed, including in thymus, lung and spleen (at protein level) . Very low levels, if any, in the brain.

Applications

ELISA, IF, ICC, WB

Field of Research

Apoptosis, Apoptotic Markers, Cancer, Caspases, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Proinflammatory caspase (PubMed:8702803, PubMed:9038361, PubMed:25119034) . Essential effector of NLRP3 inflammasome- dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS, as well as infection with Gram-negative bacteria (PubMed:22002608) . Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:22002608, PubMed:26320999, PubMed:26375003) . Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection. In later stages of the infection (>3 days post infection), LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in the pyroptosis of the infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation (PubMed:25121752) . Involved in LPS- induced IL6 secretion; this activity may not require caspase enzymatic activity (By similarity) . Involved in cell death induced by endoplasmic reticulum stress (By similarity) . Activated by binding to LPS without the need of an upstream sensor (PubMed:25119034) . Does not ly process IL1B (PubMed:8702803, PubMed:9038361) . During non-canonical inflammasome activation, cuts MB21D1 and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590) .

References & Citations

1. Kajiwara, Y., Akram, A., Katsel, P., Haroutunian, V., Schmeidler, J., Beecham, G., Haines, J. L., Pericak-Vance, M. A., Buxbaum, J. D.FE65 binds teashirt, inhibiting expression of primate-specific caspase-4. PLoS One 4:e5071, 2009. 2. Kamens, J., Paskind, M., Hugunin, M., Talanian, R. V., Allen, H., Banach, D., Bump, N., Hackett, M., Johnston, C. G., Li, P., Mankovich, J. A., Terranova, M., Ghayur, T.Identification and characterization of ICH-2, a novel member of the interleukin-1-beta-converting enzyme family of cysteine proteases.J. Biol. Chem. 270: 15250-15256, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

79686 MW

Observed Molecular Weight

43 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Caspase 4, apoptosis-related cysteine peptidase

Subcellular Location

Cytoplasm, cytosol.

Protein Name

Peroxisomal multifunctional enzyme type 2

Isotype

Rabbit IgG

Contents

243