Anti-CDC45L Antibody Picoband®

About this product

Boster Bio Anti-CDC45L Antibody Picoband® catalog # A01367-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01367-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CDC45 is a protein that in humans is encoded by the CDC45L gene. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

Synonyms

Cell division control protein 45 homolog; PORC-PI-1; CDC45; CDC45L; CDC45L2; UNQ374/PRO710

Gene Name

CDC45

Gene ID

8318

UniProt

O75419

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human CDC45L recombinant protein (Position: E166-A431) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.

Applications

ELISA, WB

Field of Research

Cell Biology, Cell Cycle, Cell Division, DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Required for initiation of chromosomal DNA replication.

References & Citations

1. Entrez Gene: CDC45L CDC45 cell division cycle 45-like (S. cerevisiae) . 2. McKie JM, Wadey RB, Sutherland HF, Taylor CL, Scambler PJ (Sep 1998) . selection of conserved cDNAs from the DiGeorge critical region: isolation of a novel CDC45-like gene. Genome Res. 8 (8) : 834–41. 3. Saha P, Thome KC, Yamaguchi R, Hou Z, Weremowicz S, Dutta A (Aug 1998) . The human homolog of Saccharomyces cerevisiae CDC45. J. Biol. Chem. 273 (29) : 18205–9.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

66 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cell division cycle 45

Subcellular Location

Cytoplasm. Nucleus.

Isotype

Rabbit IgG

Contents

243