Anti-EPO Antibody Picoband®

About this product

Boster Bio Anti-EPO Antibody Picoband® catalog # A00484-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00484-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This EPO gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.

Synonyms

Erythropoietin; Epo

Gene Name

Epo

Gene ID

13856

UniProt

P07321

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived mouse EPO recombinant protein (Position: D34-R192) .

Clonality

Polyclonal

Tissue Specificity

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Applications

WB

Field of Research

Angiogenesis, Apoptosis, Atherosclerosis, Cardiovascular, G Protein Signaling, Hematopoietic Progenitors, Hypertrophy, Immunoglobulins, Immunology, Receptors, Signal Transduction, Signaling Pathway, Small G Proteins, Stem Cells, Surface Molecules

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

References & Citations

1. Hosseini-Zare MS, Dashti-Khavidaki S, Mahdavi-Mazdeh M, Ahmadi F, Akrami S (2012) . Peripheral neuropathy response to erythropoietin in type 2 diabetic patients with mild to moderate renal failure. Clinical Neurology and Neurosurgery. 114 (6) : 663–7. 2. Middleton SA, Barbone FP, Johnson DL, Thurmond RL, You Y, McMahon FJ, Jin R, Livnah O, Tullai J, Farrell FX, Goldsmith MA, Wilson IA, Jolliffe LK (1999) . Shared and unique determinants of the erythropoietin (EPO) receptor are important for binding EPO and EPO mimetic peptide. The Journal of Biological Chemistry. 274 (20) : 14163–9.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

52221 MW

Observed Molecular Weight

28 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Erythropoietin

Subcellular Location

Secreted.

Protein Name

Eukaryotic translation initiation factor 3 subunit E

Isotype

Rabbit IgG

Contents

243