Anti-GPCR GPR40/FFAR1 Antibody Picoband®

About this product

Boster Bio Anti-GPCR GPR40/FFAR1 Antibody Picoband® catalog # A01349-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01349-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Free fatty acid receptor 1 (FFA1), also known as GPR40, is a class A G-protein coupled receptor that in humans is encoded by the FFAR1 gene. This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes.

Synonyms

Free fatty acid receptor 1; G-protein coupled receptor 40; FFAR1; GPR40

Gene Name

FFAR1

Gene ID

2864

UniProt

O14842

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GPCR GPR40, which shares 88% and 84% amino acid (aa) sequence identity with mouse and rat GPCR GPR40, respectively.

Clonality

Polyclonal

Tissue Specificity

Detected in brain and pancreas. Detected in pancreatic beta cells.

Applications

WB

Field of Research

G Protein Signaling, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose- stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1) . May also play a role in bone homeostasis; receptor signaling activates pathways that inhibit osteoclast differentiation (By similarity) . Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G (q) and G (i) -mediated pathway.

References & Citations

1. Ichimura A, Hirasawa A, Hara T, Tsujimoto G (September 2009) . Free fatty acid receptors act as nutrient sensors to regulate energy homeostasis. Prostaglandins Other Lipid Mediat. 89 (3-4) : 82–8. 2. Sum CS, Tikhonova IG, Neumann S, Engel S, Raaka BM, Costanzi S, Gershengorn MC (October 2007) . Identification of residues important for agonist recognition and activation in GPR40. J. Biol. Chem. 282 (40) : 29248–55.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

31 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Free fatty acid receptor 1

Subcellular Location

Cell membrane.

Isotype

Rabbit IgG

Contents

243