Anti-Insulin degrading enzyme/IDE Antibody Picoband®

About this product

Boster Bio Anti-Insulin degrading enzyme/IDE Antibody Picoband® catalog # A01358-2. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01358-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Insulin-degrading enzyme, also known as IDE, is an enzyme. This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Synonyms

Insulin-degrading enzyme; Abeta-degrading protease; Insulin protease; Insulinase; Insulysin; IDE

Gene Name

IDE

Gene ID

416

UniProt

P14735

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human IDE recombinant protein (Position: F485-K756) .

Clonality

Polyclonal

Applications

ELISA, Flow Cytometry, IHC, ICC, WB

Field of Research

Alzheimer's Disease, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Diabetes, Diabetes-associated, Growth Factors/Hormones, Heart Disease, Metabolism, Metalloprotease, Neurodegenerative Disease, Neurology Process, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.

References & Citations

1. Entrez Gene: IDE insulin-degrading enzyme. 2. Affholter JA, Fried VA, Roth RA (Dec 1988) . Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III. Science. 242 (4884) : 1415–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

118 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Insulin degrading enzyme

Subcellular Location

Cytoplasm. Cell membrane. Secreted. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.

Isotype

Rabbit IgG

Contents

243