Anti-Lactoferrin/LTF Antibody Picoband®

About this product

Boster Bio Anti-Lactoferrin/LTF Antibody Picoband® catalog # A00633-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00633-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides.

Synonyms

Lactotransferrin; Lactoferrin; Growth-inhibiting protein 12; Talalactoferrin; Lactoferricin-H; Lfcin-H; Kaliocin-1; Lactoferroxin-A; Lactoferroxin-B; Lactoferroxin-C; LTF; GIG12; LF

Gene Name

LTF

Gene ID

4057

UniProt

P02788

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Lactoferrin recombinant protein (Position: D529-K710) .

Clonality

Polyclonal

Tissue Specificity

High levels are found in saliva and tears, intermediate levels in serum and plasma, and low levels in urine. In kidney, detected in the distal collecting tubules in the medulla but not in the cortical region or in blood vessels. Detected in peripheral blood neutrophils (at protein level) . Isoform 1 and isoform DeltaLf are expressed in breast, prostate, spleen, pancreas, kidney, small intestine, lung, skeletal muscle, uterus, thymus and fetal liver. Isoform 1 is expressed in brain, testis and peripheral blood leukocytes; isoform DeltaLf is barely detectable in these tissues. Isoform DeltaLf is expressed in placenta, liver and ovary; isoform 1 is barely detectable in these tissues. In kidney, isoform 1 is expressed at high levels in the collecting tubules of the medulla but at very low levels in the cortex.

Applications

ELISA, IF, IHC, WB

Field of Research

Cardiovascular, Cell Death, Invasion/Microenvironment, Serum Proteins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transferrins are iron binding transport proteins which can bind two Fe (3+) ions in association with the binding of an anion, usually bicarbonate.

References & Citations

1. Jing-Fen Kang; Xiang-Long Li; Rong-Yan Zhou; Lan-Hui Li; Fu-Jun Feng; Xiu -Li Guo (2008) . Bioinformatics Analysis of Lactoferrin Gene for Several Species. Biochemical Genetics. 46 (5–6) : 312–322. 2. Sánchez L, Calvo M, Brock JH (1992) . Biological role of lactoferrin. Arch. Dis. Child. 67 (5) : 657–61.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

78 kDa

Observed Molecular Weight

85 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Lactotransferrin

Subcellular Location

Isoform 1: Secreted. Cytoplasmic granule. Secreted into most exocrine fluids by various endothelial cells. Stored in the secondary granules of neutrophils.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.