Anti-DNA Polymerase iota/POLI Antibody Picoband®

About this product

Boster Bio Anti-DNA Polymerase iota/POLI Antibody Picoband® catalog # A01376-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01376-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

DNA polymerase iota is an enzyme that in humans is encoded by the POLI gene. The protein encoded by this gene is an error-prone DNA polymerase involved in DNA repair. The encoded protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion.

Synonyms

DNA polymerase iota; Eta2; RAD30 homolog B; POLI; RAD30B

Gene Name

POLI

Gene ID

11201

UniProt

Q9UNA4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DNA Polymerase iota, which shares 70.6% and 76.5% amino acid (aa) sequence identity with mouse and rat DNA Polymerase iota, respectively.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Highly expressed in testis.

Applications

WB

Field of Research

Host-Virus Interaction, Interspecies Interaction, Microbiology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

References & Citations

1. Frank EG, Woodgate R (Aug 2007) . Increased catalytic activity and altered fidelity of human DNA polymerase iota in the presence of manganese. J Biol Chem. 282 (34) : 24689–96. 2. McDonald JP, Tissier A, Frank EG, et al. (2001) . DNA polymerase iota and related rad30-like enzymes.. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 356 (1405) : 53–60.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

83 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

DNA polymerase iota

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

243