Anti-RUNX1T1/ETO Antibody Picoband®

About this product

Boster Bio Anti-RUNX1T1/ETO Antibody Picoband® catalog # A01792-1. Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01792-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein CBFA2T1 is a protein that in humans is encoded by the RUNX1T1 gene. This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t (8;21) (q22; q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants.

Synonyms

Protein CBFA2T1; Cyclin-D-related protein; Eight twenty one protein; Protein ETO; Protein MTG8; Zinc finger MYND domain-containing protein 2; RUNX1T1; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2

Gene Name

RUNX1T1

Gene ID

862

UniProt

Q06455

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human RUNX1T1/ETO recombinant protein (Position: T335-D510) .

Clonality

Polyclonal

Tissue Specificity

Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary.

Applications

ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB

Field of Research

Cancer, Domain Families, Epigenetics and Nuclear Signaling, Oncoproteins/Suppressors, Transcription, Transcription Factors, Tumor Suppressors, Zinc Finger

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199) . Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453) . Can repress transactivation mediated by TCF12 (PubMed:16803958) . Acts as a negative regulator of adipogenesis (By similarity) . The AML1-MTG8/ETO fusion protein frequently found in leukemic cells is involved in leukemogenesis and contributes to hematopoietic stem/progenitor cell self-renewal (PubMed:23812588) .

References & Citations

1. Entrez Gene: RUNX1T1 runt-related transcription factor 1; translocated to, 1 (cyclin D-related) . 2. Calabi F, Cilli V (Dec 1998) . CBFA2T1, a gene rearranged in human leukemia, is a member of a multigene family. Genomics. 52 (3) : 332–341.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

67 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

RUNX1 translocation partner 1

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

243