Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband®

About this product

Boster Bio Anti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® catalog # A01413-1. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01413-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The SCNN1A gene encodes the alpha subunit of the epithelial sodium channel (ENaC), a constitutively active channel that allows the flow of sodium ions from the lumen into epithelial cells across the apical cell membrane. The ENaC channel, which is regulated by the renin-angiotensin-aldosterone system, has a central role in the regulation of extracellular fluid volume and blood pressure. The other subunits are encoded by the beta (SCNN1B), gamma (SCNN1G), and delta (SCNN1D) genes. This SCNN1A gene is mapped to 12p13.31. Mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids.

Synonyms

Amiloride-sensitive sodium channel subunit alpha; Alpha-NaCH; Epithelial Na (+) channel subunit alpha; Alpha-ENaC; ENaCA; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; SCNN1A; SCNN1

Gene Name

SCNN1A

Gene ID

6337

UniProt

P37088

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SCNN1A, which shares 83.3% amino acid (aa) sequence identity with both mouse and rat SCNN1A.

Clonality

Polyclonal

Tissue Specificity

Expressed in the female reproductive tract, from the fimbrial end of the fallopian tube to the endometrium (at protein level) (PubMed:22207244) . Expressed in kidney (at protein level) . In the respiratory tract, expressed in the bronchial epithelium (at protein level) . Highly expressed in lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta (PubMed:22207244) . in skin, expressed in keratinocytes, melanocytes and Merkel cells of the epidermal sub- layers, stratum basale, stratum spinosum and stratum granulosum (at protein level) (PubMed:28130590) . Expressed in the outer root sheath of the hair follicles (at protein level) (PubMed:28130590) . Detected in both peripheral and central cells of the sebaceous gland (at protein level) (PubMed:28130590) . Expressed by eccrine sweat glands (at protein level) (PubMed:28130590) . In skin, also expressed by arrector pili muscle cells and intradermal adipocytes (PubMed:28130590) . Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart (PubMed:9575806) .

Applications

IF, ICC, WB

Field of Research

Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, NK Cells, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.

References & Citations

1. Hanukoglu, I., Hanukoglu, A. Epithelial sodium channel (ENaC) family: phylogeny, structure-function, tissue distribution, and associated inherited diseases. Gene 579: 95-132, 2016. 2. Iwai, N., Baba, S., Mannami, T., Ogihara, T., Ogata, J. Association of a sodium channel alpha subunit promoter variant with blood pressure. J. Am. Soc. Nephrol. 13: 80-85, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

76 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Sodium channel epithelial 1 alpha subunit

Subcellular Location

Apical cell membrane.

Isotype

Rabbit IgG

Contents

243