Anti-BRD7 Antibody Picoband®

About this product

Boster Bio Anti-BRD7 Antibody Picoband® catalog # A01289-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01289-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Bromodomain-containing protein 7 is a protein that in humans is encoded by the BRD7 gene. This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants.

Synonyms

Bromodomain-containing protein 7; 75 kDa bromodomain protein; Protein CELTIX-1; BRD7; BP75; CELTIX1

Gene Name

BRD7

Gene ID

29117

UniProt

Q9NPI1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human BRD7 recombinant protein (Position: T514-R567) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in liver; lower levels in lung and kidney.

Applications

ELISA, IHC, WB

Field of Research

Cell Biology, Cell Cycle, Cell Division,2339, Co-Factors, Epigenetics and Nuclear Signaling, Pol II Transcription, Polymerase Associated Factors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity) . Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac) . Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.

References & Citations

1. Entrez Gene: BRD7 bromodomain containing 7. 2. Cuppen E, van Ham M, Pepers B, Wieringa B, Hendriks W (November 1999) . Identification and molecular characterization of BP75, a novel bromodomain-containing protein. FEBS Lett. 459 (3) : 291–298. 3. Kaeser MD, Aslanian A, Dong MQ, Yates JR III, Emerson BM (November 2008) . BRD7, a novel PBAF-specific SWI/SNF subunit, is required for target gene activation and repression in embryonic stem cells. J Biol Chem. 283 (47) : 32254–32263.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

74 kDa

Observed Molecular Weight

85 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Bromodomain containing 7

Subcellular Location

Isoform 2: Nucleus.

Isotype

Rabbit IgG

Contents

243