- Catalog number
- A01674-1
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog, also known as FGR, is a protein which in humans is encoded by the FGR gene. This gene is a member of the Src family of protein tyrosine kinases (PTKs) . The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified.
- Synonyms
- Tyrosine-protein kinase Fgr; Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog; Proto-oncogene c-Fgr; p55-Fgr; p58-Fgr; p58c-Fgr; FGR; SRC2
- Gene Name
- FGR
- Gene ID
- 2268
- UniProt
- P09769
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human FGR, which shares 92% amino acid (aa) sequence identity with both mouse and rat FGR.
- Clonality
- Polyclonal
- Tissue Specificity
- Detected in neutrophils, monocytes and natural killer cells (at protein level) . Detected in monocytes and large lymphocytes.
- Applications
- WB
- Field of Research
- Cancer, Protein Phosphorylation, Signal Transduction, Tyrosine Kinases
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK- dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1.
- References & Citations
- 1. Entrez Gene: FGR Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog. 2. Tronick SR, Popescu NC, Cheah MS, Swan DC, Amsbaugh SC, Lengel CR, DiPaolo JA, Robbins KC (Oct 1985) . of Sciences of the United States of America. 82 (19) : 6595–9.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 50514 MW
- Observed Molecular Weight
- 59 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- FGR proto-oncogene, Src family tyrosine kinase
- Subcellular Location
- Cell membrane ; Detected in mitochondrial intermembrane space and at inner membranes (By similarity) . Colocalizes with actin fibers at membrane ruffles. Detected at plasma membrane lipid rafts.
- Protein Name
- Carboxypeptidase M
- Isotype
- Rabbit IgG
- Contents
- 243
