Anti-CD18 ITGB2 Antibody Picoband® (monoclonal, 1A3/2A10)

About this product

Boster Bio Anti-CD18 ITGB2 Antibody Picoband® (monoclonal, 1A3/2A10) catalog # M00458-1. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00458-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ITGB2 (INTEGRIN, BETA-2), also known as CD18, is a protein that in humans is encoded by the ITGB2 gene. ITGB2 is an integrin protein that belongs to the class of cell membrane glycoproteins. The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated CD18. The ITGB2 gene is mapped to 21q22.3. The expression of CD18 is increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. In humans lack of ITGB2 causes Leukocyte Adhesion Deficiency, a disease defined by a lack of leukocyte extravasation from blood into tissues. Although ITGB2 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways.

Synonyms

Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; CD18; ITGB2; CD18; MFI7

Gene Name

ITGB2

Gene ID

3689

UniProt

P05107

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CD18 recombinant protein (Position: Q404-S769) . Human CD18 shares 76% amino acid (aa) sequence identity with mouse CD18.

Clonality

Monoclonal

Clone

Clone: 1A3/2A10

Tissue Specificity

Leukocytes.

Applications

IHC, WB

Field of Research

Cell Adhesion, Cell Type Markers, Cytoskeleton/ECM, Hematopoietic Progenitors, Immunology, Integrins, Myeloid, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha- D/beta-2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed:15356110) . Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992) . Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400) . Integrin alpha-L/beta-2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590) .

References & Citations

1. Abramson, J. S., Mills, E. L., Sawyer, M. K., Regelmann, W. R., Nelson, J. D., Quie, P. G. Recurrent infections and delayed separation of the umbilical cord in an infant with abnormal phagocytic cell locomotion and oxidative response during particle phagocytosis. J. Pediat. 99: 887-894, 1981. 2. Akao, Y., Utsumi, K. R., Naito, K., Ueda, R., Takahashi, T., Yamada, K. Chromosomal assignments of genes coding for human leukocyte common antigen, T-200, and lymphocyte function-associated antigen 1, LFA-1 beta subunit. Somat. Cell Molec. Genet. 13: 273-278, 1987. 3. Entrez Gene: ITGB2 integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

85 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Integrin beta-2

Isotype

Mouse IgG1

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.