Anti-Elafin/Skalp PI3 Antibody (monoclonal, C7)

About this product

Boster Bio Anti-Elafin/Skalp PI3 Antibody Picoband® (monoclonal, C7) catalog # M00364. Tested in ELISA, IHC applications. This antibody reacts with Human.

Specifications

Catalog number

M00364

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoprotease (SKALP), is a protein that in humans is encoded by the PI3 gene. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. And this gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.

Synonyms

Elafin; Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; PI-3; Protease inhibitor WAP3; Skin-derived antileukoproteinase; SKALP; WAP four-disulfide core domain protein 14; PI3; WAP3; WFDC14

Gene Name

PI3

Gene ID

5266

UniProt

P19957

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117) .

Clonality

Monoclonal

Clone

Clone: C7

Tissue Specificity

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Applications

ELISA (Cap), IHC

Field of Research

Cell Biology, Cell Cycle, Immunology, Innate Immunity, Kinases/Phosphatases, Lipid Signaling, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Signaling Pathway, TLR Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

References & Citations

1. Entrez Gene: PI3 peptidase inhibitor 3, skin-derived (SKALP) . 2. Clauss A, Lilja H, Lundwall A (Nov 2002) . A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein. Biochem J 368 (Pt 1) : 233–42. 3. Molhuizen HO, Zeeuwen PL, Olde Weghuis D, Geurts van Kessel A, Schalkwijk J (Feb 1994) . Assignment of the human gene encoding the epidermal serine proteinase inhibitor SKALP (PI3) to chromosome region 20q12→q13. Cytogenet Cell Genet 66 (2) : 129–31.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

25 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Peptidase inhibitor 3

Subcellular Location

Secreted.

Protein Name

Glial fibrillary acidic protein

Isotype

Mouse IgG1

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.