- Catalog number
- M00393-2
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
- Synonyms
- Interferon gamma; IFN-gamma; IFNG
- Gene Name
- IFNG
- Gene ID
- 25712
- UniProt
- P01581
- Host
- Mouse
- Reactivity
- Rat
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E. coli-derived rat Interferon gamma recombinant protein (Position: Q23-C156) . Rat Interferon gamma shares 38% and 86% amino acid (aa) sequence identity with human and mouse Interferon gamma, respectively.
- Clonality
- Monoclonal
- Clone
- Clone: Clone: 8E9
- Tissue Specificity
- Released primarily from activated T lymphocytes.
- Applications
- ELISA (Cap)
- Field of Research
- Cancer, Cytokines, Immunology, Innate Immunity, Interferons, Signaling Pathways, Stem Cells, TGF Beta, Tumor Immunology
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
- References & Citations
- 1. Nanba D, Higashiyama S (February 2004) . Dual intracellular signaling by proteolytic cleavage of membrane-anchored heparin-binding EGF-like growth factor. Cytokine Growth Factor Rev. 15 (1) : 13–9. 2. Jin K, Mao XO, Sun Y, Xie L, Jin L, Nishi E, Klagsbrun M, Greenberg DA (July 2002) . Heparin-binding epidermal growth factor-like growth factor: hypoxia-inducible expression in vitro and stimulation of neurogenesis in vitro and in vivo. J. Neurosci. 22 (13) : 5365–73. 3. Fen, Z., Dhadly, M. S., Yoshizumi, M., Hilkert, R. J., Quertermous, T., Eddy, R. L., Shows, T. B., Lee, M.-E. Structural organization and chromosomal assignment of the gene encoding the human heparin-binding epidermal growth factor-like growth factor/diphtheria toxin receptor. Biochemistry 32: 7932-7938, 1993.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Observed Molecular Weight
- 53 kDa
- Specificity
- No cross reactivity with other proteins.
- Applications Notes
- ELISA (Cap), 1-5μg/ml
- Gene Name Synonym
- Interferon, gamma
- Subcellular Location
- Secreted.
- Protein Name
- Membrane cofactor protein
- Isotype
- Mouse IgG1
- Contents
- Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
