- Catalog number
- M00516-2
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Isocitrate dehydrogenase [NADP], mitochondrialis anenzymethat in humans is encoded by theIDH2gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+) -dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+) -dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.
- Synonyms
- Serum paraoxonase/arylesterase 1; PON 1
- Gene Name
- Pon1
- Gene ID
- 18979
- UniProt
- P52430
- Host
- Mouse
- Reactivity
- Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E. coli-derived mouse PON1 recombinant protein (Position: A30-D274) .
- Clonality
- Monoclonal
- Clone
- Clone: 11H2
- Tissue Specificity
- Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.
- Applications
- ELISA (Cap)
- Field of Research
- Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Diabetes, Diabetes-associated, Drug Metabolism, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.
- References & Citations
- 1. Costa LG, Cole TB, Vitalone A, Furlong CE (2005) . Measurement of paraoxonase (PON1) status as a potential biomarker of susceptibility to organophosphate toxicity. CLINICA CHEMICA ACTA 352 (1-2) : 37–47. 2. Khateeb J, Gantman A, Kreitenberg AJ, Aviram M, Fuhrman B (January 2010) . Paraoxonase 1 (PON1) expression in hepatocytes is upregulated by pomegranate polyphenols: a role for PPAR-gamma pathway.Atherosclerosis 208 (1) : 119–25. 3. Primo-Parmo SL, Sorenson RC, Teiber J, La Du BN (May 1996) . The human serum paraoxonase/arylesterase gene (PON1) is one member of a multigene family. Genomics 33 (3) : 498–507.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Observed Molecular Weight
- 40 kDa
- Specificity
- No cross reactivity with other proteins.
- Applications Notes
- ELISA (Cap), 1-5μg/ml
- Gene Name Synonym
- Paraoxonase 1
- Subcellular Location
- Secreted, extracellular space.
- Protein Name
- Integrin beta-2
- Isotype
- Mouse IgG2a
- Contents
- Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
