Anti-Emerin EMD Antibody Picoband® (monoclonal, 5A10)

About this product

Boster Bio Anti-Emerin EMD Antibody Picoband® (monoclonal, 5A10) catalog # M00714. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00714

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

APOBEC3G (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G) is a human enzyme encoded by the APOBEC3G gene. This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.

Synonyms

Emerin; EMD; EDMD; STA

Gene Name

EMD

Gene ID

2010

UniProt

P50402

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Emerin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by nine amino acids.

Clonality

Monoclonal

Clone

Clone: 5A10

Tissue Specificity

Skeletal muscle, heart, colon, testis, ovary and pancreas.

Applications

Flow Cytometry, IHC, ICC, WB

Field of Research

Biochemicals, Chemical Type, Neuroscience, Neurotransmission, Obesity, Receptors / Channels

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta- catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.

References & Citations

1. Entrez Gene: EMD emerin (Emery–Dreifuss muscular dystrophy) . 2. Lammerding, J; Hsiao, J; Schulze, PC; Kozlov, S; Stewart, CL; Lee, RT (29 August 2005) . Abnormal nuclear shape and impaired mechanotransduction in emerin-deficient cells.. The Journal of Cell Biology. 170 (5) : 781–91. 3. Nagano A, Koga R, Ogawa M, Kurano Y, Kawada J, Okada R, Hayashi YK, Tsukahara T, Arahata K (Mar 1996) . Emerin deficiency at the nuclear membrane in patients with Emery–Dreifuss muscular dystrophy. Nature Genetics. 12 (3) : 254–9.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

34 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Emerin

Subcellular Location

Nucleus inner membrane

Isotype

Mouse IgG1

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.