Anti-IGFBP2 Antibody Picoband®

About this product

Boster Bio Anti-IGFBP2 Antibody Picoband® catalog # A01373-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01373-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP) . All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Human IGFBP-2 cDNA encodes a 328 amino acid (aa) residue precursor protein with a putative 39 aa residue signal peptide that is processed to generate the 289 aa residue mature protein. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF II, exhibiting a 2-10 fold higher affinity for IGF II than for IGF I.

Synonyms

Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2; BRL-BP; Igfbp2; Igfbp-2

Gene Name

Igfbp2

Gene ID

25662

UniProt

P12843

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived rat IGFBP2 recombinant protein (Position: E35-Q304) .

Clonality

Polyclonal

Tissue Specificity

In adults, expressed in brain, testes, ovaries, and kidney. Expression in the adult liver is barely detectable.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits IGF-mediated growth and developmental rates (By similarity) . IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

References & Citations

1. Chesik D, De Keyser J, Wilczak N (2007) . Insulin-like growth factor binding protein-2 as a regulator of IGF actions in CNS: implications in multiple sclerosis.. Cytokine Growth Factor Rev. 18 (3–4) : 267–78. 2. Wolf E, Lahm H, Wu M et al. (2000) . Effects of IGFBP-2 overexpression in vitro and in vivo..Pediatr. Nephrol. 14 (7) : 572–8. 3. Zapf J, Kiefer M, Merryweather J, Musiarz F, Bauer D, Born W, Fischer JA, Froesch ER (Oct 1990) . Isolation from adult human serum of four insulin-like growth factor (IGF) binding proteins and molecular cloning of one of them that is increased by IGF I administration and in extrapancreatic tumor hypoglycemia.J Biol Chem 265 (25) : 14892–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

40 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Insulin-like growth factor binding protein 2

Subcellular Location

Secreted.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.