Anti-COBRA1/NELFB Antibody Picoband®

About this product

Boster Bio Anti-COBRA1/NELFB Antibody Picoband® catalog # A12025-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A12025-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cofactor of BRCA1, also known as COBRA1, is a human gene that encodes NELF-B. NELF-B is a subunit of negative elongation factor (NELF), which also includes NELF-A (WHSC2), either NELF-C or NELF-D (TH1L), and NELF-E (RDBP) . NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1) and SPT5 (SUPT5H), to cause transcriptional pausing of RNA polymerase II. COBRA1 was initially identified in a yeast two-hybrid screen using the BRCT1 domain of BRCA1 as bait.

Synonyms

Negative elongation factor B; NELF-B; Cofactor of BRCA1; NELFB; COBRA1; KIAA1182

Gene Name

NELFB

Gene ID

25920

UniProt

Q8WX92

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human COBRA1 recombinant protein (Position: M1-E252) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.

Applications

ELISA, WB

Field of Research

Epigenetics and Nuclear Signaling, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.

References & Citations

1. Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, Endoh M, Yamada T, Handa H (2003) . Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex. Molecular and Cellular Biology. 23 (6) : 1863–1873. 2. Ye Q, Hu YF, Zhong H, Nye AC, Belmont AS, Li R (2001) . BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. The Journal of Cell Biology. 155 (6) : 911–922.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

66 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Negative elongation factor complex member B

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

243