Anti-AMPK beta 2 PRKAB2 Antibody Picoband® (monoclonal, 6G1)

About this product

Boster Bio Anti-AMPK beta 2 PRKAB2 Antibody Picoband® (monoclonal, 6G1) catalog # M05077. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M05077

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

5'-AMP-activated protein kinase subunit beta-2 is an enzyme that in humans is encoded by the PRKAB2 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK) . AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene.

Synonyms

5'-AMP-activated protein kinase subunit beta-2; AMPK subunit beta-2; PRKAB2

Gene Name

PRKAB2

Gene ID

5565

UniProt

O43741

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human AMPK beta 2, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

Clonality

Monoclonal

Clone

Clone: 6G1

Tissue Specificity

Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.

Applications

Flow Cytometry, IHC, ICC, WB

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by inly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) .

References & Citations

1. Entrez Gene: PRKAB2 protein kinase, AMP-activated, beta 2 non-catalytic subunit. 2. Stapleton D, Mitchelhill KI, Gao G, Widmer J, Michell BJ, Teh T, House CM, Fernandez CS, Cox T, Witters LA, Kemp BE (February 1996) . Mammalian AMP-activated protein kinase subfamily. J Biol Chem 271 (2) : 611–4.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

34 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Protein kinase, AMP-activated, beta 2 non-catalytic subunit

Subcellular Location

Cytoplasm . Nucleus .

Protein Name

RNA-binding protein Musashi homolog 1

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.