Anti-CACNA2D2 Antibody Picoband®

About this product

Boster Bio Anti-CACNA2D2 Antibody Picoband® catalog # A01560-1. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01560-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Voltage-dependent calcium channel subunit alpha2delta-2 is a protein that in humans is encoded by the CACNA2D2 gene. This gene is mapped to 3p21.31. It encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

Synonyms

Voltage-dependent calcium channel subunit alpha-2/delta-2; Voltage-gated calcium channel subunit alpha-2/delta-2; Voltage-dependent calcium channel subunit alpha-2-2; Voltage-dependent calcium channel subunit delta-2; CACNA2D2; KIAA0558

Gene Name

CACNA2D2

Gene ID

9254

UniProt

Q9NY47

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human CACNA2D2 recombinant protein (Position: Q66-Q284) .

Clonality

Polyclonal

Tissue Specificity

Predominantly present in cerebellar cortex. Present in various lung tumor cell lines, while it is absent in normal lung (at protein level) . Highly expressed in heart, lung, testis, pancreas and skeletal muscle. Also expressed in kidney, liver, placenta and brain.

Applications

ELISA, Flow Cytometry, IHC, ICC, WB

Field of Research

Apoptosis, Associated Proteins, Calcium Channels, Calcium Signaling, Cell Biology, Intracellular, Neuroscience, Neurotransmission, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G) . Overexpression induces apoptosis.

References & Citations

1. Brill, J., Klocke, R., Paul, D., Boison, D., Gouder, N., Klugbauer, N., Hofmann, F., Becker, C.-M., Becker, K.Entla, a novel epileptic and ataxic Cacna2d2 mutant of the mouse. J. Biol. Chem. 279: 7322-7330, 2004. 2. Brodbeck, J., Davies, A., Courtney, J.-M., Meir, A., Balaguero, N., Canti, C., Moss, F. J., Page, K. M., Pratt, W. S., Hunt, S. P., Barclay, J., Rees, M., Dolphin, A. C. The ducky mutation in Cacna2d2 results in altered Purkinje cell morphology and is associated with the expression of a truncated alpha-2/delta-2 protein with abnormal function. J. Biol. Chem. 277: 7684-7693, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

130 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Calcium voltage-gated channel auxiliary subunit alpha2delta 2

Subcellular Location

Membrane; Colocalizes with CACNA1A in lipid raft fractions.

Protein Name

Interleukin-1 receptor-associated kinase-like 2

Isotype

Rabbit IgG

Contents

243