Anti-Cortactin/CTTN Antibody Picoband®

About this product

Boster Bio Anti-Cortactin/CTTN Antibody Picoband® catalog # A01253-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01253-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cortactin, mapped to 11q13.3, is a multidomain protein. This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

Synonyms

Src substrate cortactin; Amplaxin; Oncogene EMS1; CTTN; EMS1

Gene Name

CTTN

Gene ID

2017

UniProt

Q14247

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Cortactin recombinant protein (Position: M1-Q105) .

Clonality

Polyclonal

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Actin Assembly, Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:21296879) . Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity) . Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity) . Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed:16636290) . Plays a role in focal adhesion assembly and turnover (By similarity) . In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P) -mediated endothelial cell (EC) barrier enhancement (PubMed:20861316) . Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782) . Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity) . Required for stabilization of KCNH1 channels at the cell membrane (PubMed:23144454) .

References & Citations

1. Tegtmeyer, N., Wittelsberger, R., Hartig, R., Wessler, S., Martinez-Quiles, N., Backert, S. Serine phosphorylation of cortactin controls focal adhesion kinase activity and cell scattering induced by Helicobacter pylori. Cell Host Microbe 9: 520-531, 2011. 2. Williams, M. R., Markey, J. C., Doczi, M. A., Morielli, A. D. An essential role for cortactin in the modulation of the potassium channel Kv1.2. Proc. Nat. Acad. Sci. 104: 17412-17417, 2007.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

85 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cortactin

Subcellular Location

Cytoplasm, cytoskeleton.

Isotype

Rabbit IgG

Contents

243