Anti-EML4 Antibody Picoband®

About this product

Boster Bio Anti-EML4 Antibody Picoband® catalog # A00930-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00930-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Echinoderm microtubule-associated protein-like 4 is a protein that in humans is encoded by the EML4gene. This gene is a member of the echinoderm microtubule associated protein-like family, and it mapped to 2p21. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants.

Synonyms

Echinoderm microtubule-associated protein-like 4; EMAP-4; Restrictedly overexpressed proliferation-associated protein; Ropp 120; EML4; C2orf2; EMAPL4

Gene Name

EML4

Gene ID

27436

UniProt

Q9HC35

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human EML4 recombinant protein (Position: K207-N423) .

Clonality

Polyclonal

Tissue Specificity

High expression in adult thyroid, lower expression in adult and fetal kidney and fetal brain. Not expressed in other tissues.

Applications

ELISA, Flow Cytometry, IF, IHC, WB

Field of Research

Cytoskeleton, Microtubules, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic. .

References & Citations

1. Heidebrecht, H. J., Buck, F., Pollmann, M., Siebert, R., Parwaresch, R. Cloning and localization of C2orf2-ropp120, a previously unknown WD repeat protein. Genomics 68: 348-350, 2000. 2. Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y.-C., Ogrodowski, P., Crippa, A., Rekhtman, N., de Stanchina, E., Lowe, S. W., Ventura, A. In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.Nature 516: 423-427, 2014. Note: Erratum: Nature 524: 502 only, 2015.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

108 kDa

Observed Molecular Weight

120 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Echinoderm microtubule associated protein like 4

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Name

Insulin-like growth factor-binding protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.