Anti-GOLPH3 Antibody Picoband®

About this product

Boster Bio Anti-GOLPH3 Antibody Picoband® catalog # A01333-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01333-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Golgi phosphoprotein 3 (GOLPH3) is a human protein encoded by GOLPH3 gene. It is mapped to 5p13.3. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

Synonyms

Golgi phosphoprotein 3; Coat protein GPP34; Mitochondrial DNA absence factor; MIDAS; GOLPH3; GPP34

Gene Name

GOLPH3

Gene ID

64083

UniProt

Q9H4A6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human GOLPH3 recombinant protein (Position: K56-K298) .

Clonality

Polyclonal

Tissue Specificity

Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.

Applications

ELISA, IF, IHC, ICC, WB

Field of Research

Subcellular Markers, Tags & Cell Markers, Vesicle Transport

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could inly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an in role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.

References & Citations

1. Bell, A. W., Ward, M. A., Blackstock, W. P., Freeman, H. N. M., Choudhary, J. S., Lewis, A. P., Chotai, D., Fazel, A., Gushue, J. N., Paiement, J., Palcy, S., Chevet, E., Lafreniere-Roula, M., Solari, R., Thomas, D. Y., Rowley, A., Bergeron, J. J. M. Proteomics characterization of abundant Golgi membrane proteins. J. Biol. Chem. 276: 5152-5165, 2001. 2. Ng, M. M., Dippold, H. C., Buschman, M. D., Noakes, C. J., Field, S. J.GOLPH3L antagonizes GOLPH3 to determine Golgi morphology. Molec. Biol. Cell 24: 796-808, 2013.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

79919 MW

Observed Molecular Weight

36 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Golgi phosphoprotein 3

Subcellular Location

Golgi apparatus, Golgi stack membrane; Phosphatidylinositol 4-phosphate-binding and oligomerization participate in the recruitment onto Golgi membranes. .

Protein Name

Protein NDRG1

Isotype

Rabbit IgG

Contents

243