Anti-Mib1/Mindbomb Antibody Picoband®

About this product

Boster Bio Anti-Mib1/Mindbomb Antibody Picoband® catalog # A01387-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01387-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

E3 ubiquitin-protein ligase MIB1 is an enzyme that in humans is encoded by the MIB1 gene. It is mapped to 18q11.2. This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1) .

Synonyms

E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; DIP-1; Mind bomb homolog 1; RING-type E3 ubiquitin transferase MIB1 ; Zinc finger ZZ type with ankyrin repeat domain protein 2; MIB1; DIP1; KIAA1323; ZZANK2

Gene Name

MIB1

Gene ID

57534

UniProt

Q86YT6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Mib1 Mindbomb recombinant protein (Position: H465-E732) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.

Applications

ELISA, WB

Field of Research

Cytoplasmic, Signaling Pathways, Stem Cells, Surface Molecules

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis (By similarity) . Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.

References & Citations

1. Barsi, J. C., Rajendra, R., Wu, J. I., Artzt, K. Mind bomb1 is a ubiquitin ligase essential for mouse embryonic development and Notch signaling. Mech. Dev. 122: 1106-1117, 2005. 2. Henshall, D. C., Schindler, C. K., So, N. K., Lan, J.-Q., Meller, R., Simon, R. P.Death-associated protein kinase expression in human temporal lobe epilepsy. Ann. Neurol. 55: 485-494, 2004. 3. Jin, Y., Blue, E. K., Dixon, S., Shao, Z., Gallagher, P. J. A death-associated protein kinase (DAPK) -interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK. J. Biol. Chem. 277: 46980-46986, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33899 MW

Observed Molecular Weight

110 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Mindbomb E3 ubiquitin protein ligase 1

Subcellular Location

Cytoplasm.

Protein Name

Arylamine N-acetyltransferase 1

Isotype

Rabbit IgG

Contents

243