Anti-TRPM7 Antibody Picoband®

About this product

Boster Bio Anti-TRPM7 Antibody Picoband® catalog # A00789-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00789-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transient receptor potential cation channel, subfamily M, member 7, also known as TRPM7, is a human gene encoding a protein of the same name. It is mapped to 15q21.2. This gene belongs to the melastatin subfamily of transient receptor potential family of ion channels. The protein encoded by this gene is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. The encoded protein is involved in cytoskeletal organization, cell adhesion, cell migration and organogenesis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam. The gene may also be associated with defects of cardiac function.

Synonyms

Transient receptor potential cation channel subfamily M member 7; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; TRPM7; CHAK1; LTRPC7

Gene Name

TRPM7

Gene ID

54822

UniProt

Q96QT4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human TRPM7 recombinant protein (Position: K777-K905) .

Clonality

Polyclonal

Tissue Specificity

Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.

Applications

ELISA, Flow Cytometry, IF, ICC, WB

Field of Research

Intracellular Signaling, Kinases, Neurodegenerative Disease, Neurology Process, Neuroscience, Neurotransmission, Receptors / Channels

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF- induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1) .

References & Citations

1. Aarts, M., Iihara, K., Wei, W.-L., Xiong, Z.-G., Arundine, M., Cerwinski, W., MacDonald, J. F., Tymianski, M. A key role for TRPM7 channels in anoxic neuronal death. Cell 115: 863-877, 2003. 2. Farooqi, A. A., Javeed, M. K., Javed, Z., Riaz, A. M., Mukhtar, S., Minhaj, S., Abbas, S., Bhatti, S.TRPM channels: same ballpark, different players, and different rules in immunogenetics.Immunogenetics 63: 773-787, 2011. 3. Hara, K., Kokubo, Y., Ishiura, H., Fukuda, Y., Miyashita, A., Kuwano, R., Sasaki, R., Goto, J., Nishizawa, M., Kuzuhara, S., Tsuji, S. TRPM7 is not associated with amyotrophic lateral sclerosis-parkinsonism dementia complex in the Kii peninsula of Japan. Am. J. Med. Genet. 153B: 310-313, 2010.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

59216 MW

Observed Molecular Weight

250 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Transient receptor potential cation channel subfamily M member 7

Subcellular Location

Membrane; Multi-pass membrane protein.

Isotype

Rabbit IgG

Contents

243