Anti-ACTN3 Antibody Picoband® (monoclonal, 9B5)

About this product

Boster Bio Anti-ACTN3 Antibody Picoband® (monoclonal, 9B5) catalog # M02693. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M02693

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.

Synonyms

Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein; ACTN3

Gene Name

ACTN3

Gene ID

89

UniProt

Q08043

Host

Mouse

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3, different from the related mouse sequence by five amino acids.

Clonality

Monoclonal

Clone

Clone: 9B5

Tissue Specificity

Expressed only in a subset of type 2 skeletal muscle fibers.

Applications

IHC, WB

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

References & Citations

1. Papadimitriou ID, Papadopoulos C, Kouvatsi A, Triantaphyllidis C (April 2008) . The ACTN3 gene in elite Greek track and field athletes. Int J Sports Med 29 (4) : 352–5. 2. Yang N, MacArthur DG, Gulbin JP, Hahn AG, Beggs AH, Easteal S, North K (September 2003) . ACTN3 genotype is associated with human elite athletic performance. Am. J. Hum. Genet. 73 (3) : 627–31.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

103 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Actinin alpha 3 (gene/pseudogene)

Isotype

Mouse IgG1

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.