Anti-SI Antibody Picoband®

About this product

Boster Bio Anti-SI Antibody Picoband® catalog # A04542-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04542-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene is mapped to 3q26.1. It encodes a sucrase-isomaltase enzyme that is expressed in the intestinal brush border. The encoded protein is synthesized as a precursor protein that is cleaved by pancreatic proteases into two enzymatic subunits sucrase and isomaltase. These two subunits heterodimerize to form the sucrose-isomaltase complex. This complex is essential for the digestion of dietary carbohydrates including starch, sucrose and isomaltose. Mutations in this gene are the cause of congenital sucrase-isomaltase deficiency.

Synonyms

Sucrase-isomaltase, intestinal; Sucrase; Isomaltase; SI

Gene Name

SI

Gene ID

6476

UniProt

P14410

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SI, which shares 86.1% amino acid (aa) sequence identity with rat SI.

Clonality

Polyclonal

Tissue Specificity

Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.

Applications

Flow Cytometry, IHC, WB

Field of Research

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays an important role in the final stage of carbohydrate digestion. Isomaltase activity is specific for both alpha-1,4- and alpha-1,6-oligosaccharides.

References & Citations

1. Birkenmeier, E. H., Rowe, L. B., Leeper, L. L., Henning, S. J. The sucrase-isomaltase structural gene (Si-s) and a regulatory gene (Si-r) are closely linked to esterase-26 (Es-26) on mouse chromosome 3. Mammalian Genome 4: 531-536, 1993. 2. Chantret, I., Lacasa, M., Chevalier, G., Ruf, J., Islam, I., Mantei, N., Edwards, Y., Swallow, D., Rousset, M. Sequence of the complete cDNA and the 5-prime structure of the human sucrase-isomaltase gene: possible homology with a yeast glucoamylase. Biochem. J. 285: 915-923, 1992. 3. Davis, M. B., Green, F. R., West, L. F., Kearney, A., Povey, S., Swallow, D. M. Mapping of the gene encoding human sucrase-isomaltase (SI) to chromosome 3q25-26. (Abstract) Cytogenet. Cell Genet. 46: 604, 1987.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

209 kDa

Observed Molecular Weight

209 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Sucrase-isomaltase

Subcellular Location

Apical cell membrane. Single-pass type II membrane protein.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.