Anti-CDK8 Antibody Picoband®

About this product

Boster Bio Anti-CDK8 Antibody Picoband® catalog # A01493-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01493-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cell division protein kinase 8 is an enzyme that in humans is encoded by the CDK8 gene. It is mapped to 13q12.13. This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.

Synonyms

Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35; CDK8

Gene Name

CDK8

Gene ID

1024

UniProt

P49336

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Cdk8 recombinant protein (K355-Y464) .

Clonality

Polyclonal

Tissue Specificity

Predominantly expressed in the liver (PubMed:11099417, PubMed:11452359) . Low expression levels in the small intestine and colon (PubMed:11099417) . Very low levels in other tissues, including brain, heart and spleen (PubMed:11452359) .

Applications

ELISA, WB

Field of Research

Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases, Mediator Complex, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.

References & Citations

1. Akoulitchev, S., Chuikov, S., Reinberg, D. TFIIH is negatively regulated by cdk8-containing mediator complexes. Nature 407: 102-106, 2000. 2. Donner, A. J., Szostek, S., Hoover, J. M., Espinosa, J. M. CDK8 is a stimulus-specific positive coregulator of p53 target genes. Molec. Cell 27: 121-133, 2007. 3. Firestein, R., Bass, A. J., Kim, S. Y., Dunn, I. F., Silver, S. J., Guney, I., Freed, E., Ligon, A. H., Vena, N., Ogino, S., Chheda, M. G., Tamayo, P., and 16 others. CDK8 is a colorectal cancer oncogene that regulates beta-catenin activity. Nature 455: 547-551, 2008.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

75679 MW

Observed Molecular Weight

53 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cyclin dependent kinase 8

Subcellular Location

Nucleus.

Protein Name

ATP-binding cassette sub-family G member 8

Isotype

Rabbit IgG

Contents

243