Anti-Leucyl tRNA synthetase /LARS Antibody Picoband®

About this product

Boster Bio Anti-Leucyl tRNA synthetase /LARS Antibody Picoband® catalog # A01109. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01109

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Leucyl-tRNA synthetase, cytoplasmic is an enzyme that in humans is encoded by the LARS gene. This gene is mapped to 5q32. It encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA (Leu) . It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed.

Synonyms

Leucine--tRNA ligase, cytoplasmic; Leucyl-tRNA synthetase; LeuRS; LARS; KIAA1352

Gene Name

LARS

Gene ID

51520

UniProt

Q9P2J5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Leucyl tRNA synthetase/LARS recombinant protein (Q628-Q855) .

Clonality

Polyclonal

Tissue Specificity

Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]) . Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease.

Applications

ELISA, WB

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, Translation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.

References & Citations

1. Casey, J. P., McGettigan, P., Lynam-Lennon, N., McDermott, M., Regan, R., Conroy, J., Bourke, B., O'Sullivan, J., Crushell, E., Lynch, S., Ennis, S. Identification of a mutation in LARS as a novel cause of infantile hepatopathy. Molec. Genet. Metab. 106: 351-358, 2012. 2. Dana, S., Wasmuth, J. J. Selective linkage disruption in human-Chinese hamster cell hybrids: deletion mapping of the leuS, hexB, emtB, and chr genes on human chromosome 5. Molec. Cell. Biol. 2: 1220-1228, 1982. 3. Gerken, S. C., Wasmuth, J. J., Arfin, S. M. Threonyl-rRNA synthetase gene maps close to leucyl-tRNA synthetase gene on human chromosome 5. Somat. Cell Molec. Genet. 12: 519-522, 1986.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

51735 MW

Observed Molecular Weight

134 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Leucyl-tRNA synthetase

Subcellular Location

Cytoplasm.

Protein Name

Putative Polycomb group protein ASXL1

Isotype

Rabbit IgG

Contents

243