Anti-Plastin L/LCP1 Antibody Picoband®

About this product

Boster Bio Anti-Plastin L/LCP1 Antibody Picoband® catalog # A03361. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03361

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Plastin-2 is a protein that in humans is encoded by the LCP1 gene. This gene is mapped to 13q14.13. Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.) . However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues.

Synonyms

Plastin-2; L-plastin; LC64P; Lymphocyte cytosolic protein 1; LCP-1; LCP1; PLS2

Gene Name

LCP1

Gene ID

3936

UniProt

P13796

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Plastin L/LCP1 recombinant protein (M1-K444) .

Clonality

Polyclonal

Tissue Specificity

Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Actin Binding Proteins, Actin, etc., Cancer, Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction, Tumor Antigens, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.

References & Citations

1. Chen, H., Mocsai, A., Zhang, H., Ding, R.-X., Morisaki, J. H., White, M., Rothfork, J. M., Heiser, P., Colucci-Guyon, E., Lowell, C. A., Gresham, H. D., Allen, P. M., Brown, E. J. Role for plastin in host defense distinguishes integrin signaling from cell adhesion and spreading. Immunity 19: 95-104, 2003. 2. Galiegue-Zouitina, S., Quief, S., Hildebrand, M.-P., Denis, C., Detourmignies, L., Lai, J.-L., Kerckaert, J.-P. Nonrandom fusion of L-plastin (LCP1) and LAZ3 (BCL6) genes by t (3;13) (q27; q14) chromosome translocation in two cases of B-cell non-Hodgkin lymphoma. Genes Chromosomes Cancer 26: 97-105, 1999. 3. Giometti, C. S., Anderson, N. L. A variant of human nonmuscle tropomyosin found in fibroblasts by using two-dimensional electrophoresis. J. Biol. Chem. 256: 11840-11846, 1981.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

70 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Lymphocyte cytosolic protein 1

Subcellular Location

Ruffle membrane, Peripheral membrane protein, Cytoplasmic side, Cytoskeleton, Cell junction, Cell projection.

Isotype

Rabbit IgG

Contents

243