Anti-ZP3 Antibody Picoband®

About this product

Boster Bio Anti-ZP3 Antibody Picoband® catalog # A03543-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03543-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Zona pellucida sperm-binding protein 3, also known as zona pellucida glycoprotein 3 (Zp-3) or the sperm receptor, is a ZP module-containing protein that in humans is encoded by the ZP3 gene. It is mapped to 7q11.23. he zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.

Synonyms

Zona pellucida sperm-binding protein 3; Sperm receptor; ZP3A/ZP3B; Zona pellucida glycoprotein 3; Zp-3; Zona pellucida protein C; ZP3; ZP3A; ZP3B; ZPC

Gene Name

ZP3

Gene ID

7784

UniProt

P21754

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ZP3, which shares 77.4% amino acid (aa) sequence identity with both mouse and rat ZP3.

Clonality

Polyclonal

Tissue Specificity

Oocytes.

Applications

WB

Field of Research

Cell Biology, Cell Type Markers, Developmental Biology, Reproduction, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

References & Citations

1. Chen, T., Bian, Y., Liu, X., Zhao, S., Wu, K., Yan, L., Li, M., Yang, Z., Liu, H., Zhao, H., Chen, Z.-J. A recurrent missense mutation in ZP3 causes empty follicle syndrome and female infertility. Am. J. Hum. Genet. 101: 459-465, 2017. 2. Dean, J. Biology of mammalian fertilization: role of the zona pellucida. J. Clin. Invest. 89: 1055-1059, 1992. 3. Kipersztok, S., Osawa, G. A., Liang, L. F., Modi, W. S., Dean, J. POM-ZP3, a bipartite transcript derived from human ZP3 and POM121 homologue. Genomics 25: 354-359, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

75187 MW

Observed Molecular Weight

47 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Zona pellucida glycoprotein 3

Subcellular Location

Extracellular matrix, Cell membrane, Single-pass type I membrane protein.

Protein Name

Segment polarity protein dishevelled homolog DVL-1

Isotype

Rabbit IgG

Contents

243