Anti-PARN Antibody Picoband® (monoclonal, 11D8)

About this product

Boster Bio Anti-PARN Antibody Picoband® (monoclonal, 11D8) catalog # M01501. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01501

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Poly (A) -specific ribonuclease (PARN), also known as polyadenylate-specific ribonuclease or deadenylating nuclease (DAN), is an enzyme that in humans is encoded by the PARN gene. The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly (A) as the substrate, hence, efficiently degrades poly (A) tails of mRNAs. Exonucleolytic degradation of the poly (A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons.

Synonyms

Poly (A) -specific ribonuclease PARN; Deadenylating nuclease; Deadenylation nuclease; Polyadenylate-specific ribonuclease; PARN; DAN

Gene Name

PARN

Gene ID

5073

UniProt

O95453

Host

Mouse

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human PARN recombinant protein (Position: M1-Y301) .

Clonality

Monoclonal

Clone

Clone: 11D8

Tissue Specificity

Ubiquitous.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, RNA Processing

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

3'-exoribonuclease that has a preference for poly (A) tails of mRNAs, thereby efficiently degrading poly (A) tails. Exonucleolytic degradation of the poly (A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly (A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly (A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly (A) tails of AREs mRNAs, which constitutes the first step of destabilization. Also able to recognize and trim poly (A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to microRNAs degradation or snoRNA increased stability

References & Citations

1.Entrez Gene: PARN poly (A) -specific ribonuclease (deadenylation nuclease) . Buiting K, Korner C, Ulrich B, Wahle E, Horsthemke B (May 2000) . The human gene for the poly (A) -specific ribonuclease (PARN) maps to 16p13 and has a truncated copy in the Prader-Willi/Angelman syndrome region on 15q11→q13. Cytogenet Cell Genet. 87 (1–2) : 125–31.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

78 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Poly (A) -specific ribonuclease

Subcellular Location

Nucleus. Nucleolus. Cytoplasm.

Protein Name

CD79b molecule

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.