Anti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7)

About this product

Boster Bio Anti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) catalog # M00179-1. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00179-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CAV1 (Caveolin-1) is a protein that in humans is encoded by the CAV1 gene. The CAV1 gene is mapped to 7q31.2. The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.

Synonyms

Caveolin-1; CAV1; CAV

Gene Name

CAV1

Gene ID

857

UniProt

Q03135

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178) . Human Caveolin-1 shares 95% and 94% amino acid (aa) sequence identity with mouse and rat Caveolin-1, respectively.

Clonality

Monoclonal

Clone

Clone: 12C7

Tissue Specificity

Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level) . Expressed in the brain.

Applications

IF, IHC, IHC-F, WB

Field of Research

Calcium Channels, Calcium Signaling, Cancer, Cancer Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Neuroscience, Neurotransmission, Pathways and Processes, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

May act as a scaffolding protein within caveolar membranes. Interacts ly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR) -mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae.

References & Citations

1. Tahir, S. A., Yang, G., Ebara, S., Timme, T. L., Satoh, T., Li, L., Goltsov, A., Ittmann, M., Morrisett, J. D., Thompson, T. C.Secreted caveolin-1 stimulates cell survival/clonal growth and contributes to metastasis in androgen-insensitive prostate cancer.Cancer Res. 61: 3882-3885, 2001. 2. Wang, X. M., Zhang, Y., Kim, H. P., Zhou, Z., Feghali-Bostwick, C. A., Liu, F., Ifedigbo, E., Xu, X., Oury, T. D., Kaminski, N., Choi, A. M. K.Caveolin-1: a critical regulator of lung fibrosis in idiopathic pulmonary fibrosis.J. Exp. Med. 203: 2895-2906, 2006. 3. Yang, G., Truong, L. D., Timme, T. L., Ren, C., Wheeler, T. M., Park, S. H., Nasu, Y., Bangma, C. H., Kattan, M. W., Scardino, P. T., Thompson, T. C.Elevated expression of caveolin is associated with prostate and breast cancer.Clin. Cancer Res. 4: 1873-1880, 1998.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

46659 MW

Observed Molecular Weight

22 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Caveolin 1, caveolae protein, 22kDa

Subcellular Location

Cell membrane. Peripheral membrane protein. Golgi apparatus membrane. Peripheral membrane protein. Trans-Golgi network. Caveola. Peripheral membrane protein. Membrane raft.

Protein Name

Isocitrate dehydrogenase [NADP] cytoplasmic

Isotype

Mouse IgG2a

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.