Anti-NFIA Antibody Picoband® (monoclonal, 16H11)

About this product

Boster Bio Anti-NFIA Antibody Picoband® (monoclonal, 16H11) catalog # M03531. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M03531

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nuclear factor 1 A-type?is a?protein?that in?humans?is encoded?by the?NFIA?gene. Nuclear factor I (NFI) proteins constitute a family of dimericDNA-binding proteins?with similar, and possibly identical, DNA-binding specificity. They function as cellular?transcription factors?and as replication factors for?adenovirus?DNA replication. Diversity in this protein family is generated by multiple?genes, differential?splicing, and heterodimerization.

Synonyms

Nuclear factor 1 A-type; NF1-A; Nuclear factor 1/A; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein; NFIA; KIAA1439

Gene Name

NFIA

Gene ID

4774

UniProt

Q12857

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NFIA, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Clonality

Monoclonal

Clone

Clone: 16H11

Tissue Specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Epigenetics and Nuclear Signaling, Neurogenesis, Neurology Process, Neuroscience, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

References & Citations

1.Leahy P, Crawford DR, Grossman G, et al. (1999) . CREB binding protein coordinates the function of multiple transcription factors including nuclear factor I to regulate phosphoenolpyruvate carboxykinase (GTP) gene transcription.. J. Biol. Chem. 274 (13) : 8813–22. Qian F, Kruse U, Lichter P, Sippel AE (Dec 1995) . Chromosomal localization of the four genes (NFIA, B, C, and X) for the human transcription factor nuclear factor I by FISH. Genomics 28 (1) : 66–73.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

62 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Nuclear factor I A

Subcellular Location

Nucleus

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.