Anti-DHFR Antibody Picoband® (monoclonal, 3C8)

About this product

Boster Bio Anti-DHFR Antibody Picoband® (monoclonal, 3C8) catalog # M00813-1. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00813-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

SHC, also known as SHC1 (SHC-transforming protein 1) or SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms

Dihydrofolate reductase; DHFR

Gene Name

DHFR

Gene ID

1719

UniProt

P00374

Host

Mouse

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human DHFR recombinant protein (Position: V2-D187) . Human DHFRshares90%aminoacid (aa) sequenceidentitywith both mouseandrat DHFR.

Clonality

Monoclonal

Clone

Clone: 3C8

Tissue Specificity

Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.

Applications

WB, IHC

Field of Research

Amino Acid Metabolism, Amino Acids, Cancer, Cell Biology, Metabolic Signaling Pathways, Metabolism, Nucleotide Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

References & Citations

1.Chen MJ, Shimada T, Moulton AD, Harrison M, Nienhuis AW (December 1982) . Intronless human dihydrofolate reductase genes are derived from processed RNA molecules. Proc. Natl. Acad. Sci. U.S.A. 79 (23) : 7435–9. 2.Chen MJ, Shimada T, Moulton AD, Cline A, Humphries RK, Maizel J, Nienhuis AW (March 1984) . The functional human dihydrofolate reductase gene. J. Biol. Chem. 259 (6) : 3933–43. 3.Funanage VL, Myoda TT, Moses PA, Cowell HR (October 1984) . Assignment of the human dihydrofolate reductase gene to the q11----q22 region of chromosome 5. Mol. Cell. Biol. 4 (10) : 2010–6.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

22 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Dihydrofolate reductase

Subcellular Location

Mitochondrion. Cytoplasm

Protein Name

Zeta chain of T cell receptor associated protein kinase 70

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.