- Catalog number
- M05865-1
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Golgin subfamily A member 2 is a protein that in humans is encoded by the GOLGA2 gene. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs) . Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
- Synonyms
- Golgin subfamily A member 2; 130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; Golgin-95; GOLGA2
- Gene Name
- GOLGA2
- Gene ID
- 2801
- UniProt
- Q08379
- Host
- Mouse
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E.coli-derived human GM130 recombinant protein (Position: E796-E913) .
- Clonality
- Monoclonal
- Clone
- Clone: 4G3
- Tissue Specificity
- Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
- Applications
- Flow Cytometry, IF, IHC, ICC, WB
- Field of Research
- Golgi Proteins, Organelles, Protein Trafficking, Signal Transduction, Subcellular Markers
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
- Function
- Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) . Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity) . Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity) . Also plays a key role in spindle pole assembly and centrosome organization. Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle . Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity) . Also regulates the centrosome organization . Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins.
- References & Citations
- 1.Matsuki, T., Matthews, R. T., Cooper, J. A., van der Brug, M. P., Cookson, M. R., Hardy, J. A., Olson, E. C., Howell, B. W. Reelin and Stk25 have opposing roles in neuronal polarization and dendritic Golgi deployment. Cell 143: 826-836, 2010. Wong, M., Munro, S. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins. Science 346: 601 only, 2014.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Observed Molecular Weight
- 140 kDa
- Specificity
- No cross reactivity with other proteins.
- Applications Notes
- 6
- Gene Name Synonym
- Golgin A2
- Subcellular Location
- Cis-Golgi network membrane. Peripheral membrane protein. Cytoplasmic side. spindle pole. Endoplasmic reticulum-Golgi intermediate compartment membrane. Peripheral membrane protein. Cytoplasmic side.
- Isotype
- Mouse IgG2b
- Contents
- Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
