Anti-APOBEC3G Antibody Picoband® (monoclonal, 6C2)

About this product

Boster Bio Anti-APOBEC3G Antibody Picoband® (monoclonal, 6C2) catalog # M00708. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00708

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

APOBEC3G (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G) is a human enzyme encoded by the APOBEC3G gene. This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.

Synonyms

DNA dC->dU-editing enzyme APOBEC-3G; APOBEC-related cytidine deaminase; APOBEC-related protein; ARCD; APOBEC-related protein 9; ARP-9; CEM-15; CEM15; Deoxycytidine deaminase; A3G; APOBEC3G; MDS019

Gene Name

APOBEC3G

Gene ID

60489

UniProt

Q9HC16

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human APOBEC3G recombinant protein (Position: E191-N384) .

Clonality

Monoclonal

Clone

Clone: 6C2

Tissue Specificity

Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines. Exists only in the LMM form in peripheral blood-derived resting CD4 T-cells and monocytes, both of which are refractory to HIV-1 infection. LMM is converted to a HMM complex when resting CD4 T-cells are activated or when monocytes are induced to differentiate into macrophages. This change correlates with increased susceptibility of these cells to HIV-1 infection.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Antiviral Signaling, Chromatin Binding Proteins, Chromatin Modifying Enzymes, DNA/RNA Binding, Epigenetics and Nuclear Signaling, Host-Virus Interaction, Immune System Diseases, Immunology, Interspecies Interaction, Microbiology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double-stranded RNA. Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV) . May inhibit the mobility of LTR and non-LTR retrotransposons.

References & Citations

1.Chelico L, Prochnow C, Erie DA, Chen XS, Goodman MF (2011) .Structural model for deoxycytidine deaminaton mechanism of the HIV-1 inactivation enzyme APOBEC3G. The Journal of Biological Chemistry 285 (21) : 16195–205. 2.Li X, Ma J, Zhang Q, Yin X, Zhai C, You X, Yu L, Guo F, Zhao L, Li Z, Zeng Y, Cen S (2011) . Functional analysis of the two cytidine deaminase domains in APOBEC3G. Virology 414 (2) : 130–136.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

46 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Apolipoprotein B mRNA editing enzyme catalytic subunit 3G

Subcellular Location

Nucleus. Cytoplasm. P-body.

Isotype

Mouse IgG1

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.