Anti-ATP citrate lyase ACLY Antibody Picoband® (monoclonal, 5I2)

About this product

Boster Bio Anti-ATP citrate lyase ACLY Antibody Picoband® (monoclonal, 5I2) catalog # M02372-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M02372-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.

Synonyms

ATP-citrate synthase; ATP-citrate (pro-S-) -lyase; ACL; Citrate cleavage enzyme; ACLY

Gene Name

ACLY

Gene ID

47

UniProt

P53396

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180) . Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase.

Clonality

Monoclonal

Clone

Clone: 5I2

Tissue Specificity

Widely expressed. Highest level in placenta, liver, muscle and kidney.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Cancer Metabolism, Energy Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

References & Citations

1.Fatland BL, Ke J, Anderson MD, Mentzen WI, Cui LW, Allred CC, Johnston JL, Nikolau BJ, Wurtele ES (October 2002) . Molecular characterization of a heteromeric ATP-citrate lyase that generates cytosolic acetyl-coenzyme A in Arabidopsis. Plant Physiol. 130 (2) : 740–756. 2.Guay C, Madiraju SR, Aumais A, Joly E, Prentki M (December 2007) . A role for ATP-citrate lyase, malic enzyme, and pyruvate/citrate cycling in glucose-induced insulin secretion. J. Biol. Chem. 282 (49) : 35657–35665.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

121 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

ATP citrate lyase

Subcellular Location

Cytoplasm.

Protein Name

Mitofusin-1

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.