Anti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9)

About this product

Boster Bio Anti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9) catalog # M00788. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00788

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene. It is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it is the major ligand of GPR56 in the developing brain. COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.

Synonyms

Collagen alpha-1 (III) chain; COL3A1

Gene Name

COL3A1

Gene ID

1281

UniProt

P02461

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222- E1455) .

Clonality

Monoclonal

Clone

Clone: 9H9

Tissue Specificity

Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.

Applications

WB, IHC

Field of Research

Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

References & Citations

1.Janeczko RA, Ramirez F (Oct 1989) . Nucleotide and amino acid sequences of the entire human alpha 1 (III) collagen. Nucleic Acids Res 17 (16) : 6742. 2.Superti-Furga A, Gugler E, Gitzelmann R, Steinmann B (Jun 1988) . Ehlers-Danlos syndrome type IV: a multi-exon deletion in one of the two COL3A1 alleles affecting structure, stability, and processing of type III procollagen. J Biol Chem 263 (13) : 6226–32.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

180-190 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Collagen, type III, alpha 1

Subcellular Location

Extracellular matrix.

Protein Name

Zeta chain of T cell receptor associated protein kinase 70

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.