Anti-ARP2/3 subunit 1B/ARPC1B Antibody Picoband®

About this product

Boster Bio Anti-ARP2/3 subunit 1B/ARPC1B Antibody Picoband® catalog # A07192-2. Tested in ELISA, Flow Cytometry, IP, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A07192-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Actin-related protein 2/3 complex subunit 1B is a protein that in humans is encoded by the ARPC1B gene. It is mapped to 7q22.1. This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. This protein also has a role in centrosomal homeostasis by being an activator and substrate of the Aurora A kinase.

Synonyms

Actin-related protein 2/3 complex subunit 1B; Arp2/3 complex 41 kDa subunit; p41-ARC; ARPC1B; ARC41

Gene Name

ARPC1B

Gene ID

10095

UniProt

O15143

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ARP2/3 subunit 1B/ARPC1B recombinant protein (Position: Q293-K372) .

Clonality

Polyclonal

Tissue Specificity

Isoform 1 is expressed in the jejunum, ileum, cecum and ascending colon intestine. Isoform 2 is xpressed in the gallbladder, duodenum, jejunum, ileum, cecum, ascending, transverse and descending colon, sigmoid colon and rectum. Isoform 2 is expressed in colorectal adenocarcinomas and their adjacent normal mucosa.

Applications

ELISA, Flow Cytometry, IP, IHC, WB

Field of Research

Actin Assembly, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) . The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) .

References & Citations

1. Kahr, W. H. A., Pluthero, F. G., Elkadri, A., Warner, N., Drobac, M., Chen, C. H., Lo, R. W., Li, L., Li, R., Li, Q., Thoeni, C., Pan, J., and 10 others. Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease. Nature Commun. 8: 14816, 2017. Note: Electronic Article. 2. Laurila, E., Savinainen, K., Kuuselo, R., Karhu, R., Kallioniemi, A. Characterization of the 7q21-q22 amplicon identifies ARPC1A, a subunit of the Arp2/3 complex, as a regulator of cell migration and invasion in pancreatic cancer. Genes Chromosomes Cancer 48: 330-339, 2009.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

41 kDa

Observed Molecular Weight

37 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Actin related protein 2/3 complex subunit 1B

Subcellular Location

Cytoskeleton. Nucleus.

Protein Name

Metalloproteinase inhibitor 4

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.