Anti-Plasminogen/PLG Antibody Picoband®

About this product

Boster Bio Anti-Plasminogen/PLG Antibody Picoband® catalog # A00702-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00702-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Plasmin is an important enzyme present in blood that degrades many blood plasma proteins, including fibrin clots. It is mapped to 6q26. The degradation of fibrin is termed fibrinolysis. In humans, the plasmin protein is encoded by the PLG gene. The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Plasminogen; PLG

Gene Name

PLG

Gene ID

5340

UniProt

P00747

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Plasminogen/PLG recombinant protein (Position: V98-P356) .

Clonality

Polyclonal

Tissue Specificity

Present in plasma and many other extracellular fluids. It is synthesized in the liver.

Applications

ELISA, WB

Field of Research

Cardiovascular, Cell Biology, Platelets, Protease Inhibitors, Proteolysis/Ubiquitin, Proteolytic Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.

References & Citations

1. Forsgren, M., Raden, B., Israelsson, M., Larsson, K., Heden, L.-O. Molecular cloning and characterization of a full-length cDNA clone for human plasminogen. FEBS Lett. 213: 254-260, 1987. 2. Miyata, T., Iwanaga, S., Sakata, Y., Aoki, N. Plasminogen Tochigi: inactive plasmin resulting from replacement of alanine-600 by threonine in the active site. Proc. Nat. Acad. Sci. 79: 6132-6136, 1982.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42982 MW

Observed Molecular Weight

90-100 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Plasminogen

Subcellular Location

Secreted.

Protein Name

CAMP-dependent protein kinase type I-alpha regulatory subunit

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.