Anti-RNF123 Antibody Picoband®

About this product

Boster Bio Anti-RNF123 Antibody Picoband® catalog # A09642-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A09642-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

E3 ubiquitin-protein ligase RNF123 is an enzyme that in humans is encoded by the RNF123 gene. It is mapped to 3p21.31. The protein encoded by this gene contains a C-terminal RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions, and an N-terminal SPRY domain. This protein displays E3 ubiquitin ligase activity toward the cyclin-dependent kinase inhibitor 1B which is also known as p27 or KIP1. Alternative splicing results in multiple transcript variants.

Synonyms

E3 ubiquitin-protein ligase RNF123; Kip1 ubiquitination-promoting complex protein 1; RING finger protein 123; RING-type E3 ubiquitin transferase RNF123; RNF123; KPC1; FP1477

Gene Name

RNF123

Gene ID

63891

UniProt

Q5XPI4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RNF123 recombinant protein (Position: R12-Y188) .

Clonality

Polyclonal

Tissue Specificity

Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Applications

ELISA, IHC, WB

Field of Research

Cell Biology, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ring Finger E3 Ligase, Ubiquitin E3 Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.

References & Citations

1. Kamura, T., Hara, T., Matsumoto, M., Ishida, N., Okumura, F., Hatakeyama, S., Yoshida, M., Nakayama, K., Nakayama, K. I. Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27 (Kip1) at G1 phase. Nature Cell Biol. 6: 1229-1235, 2004. 2. Lu, Y., Adegoke, O. A. J., Nepveu, A., Nakayama, K. I., Bedard, N., Cheng, D., Peng, J., Wing, S. S. USP19 deubiquitinating enzyme supports cell proliferation by stabilizing KPC1, a ubiquitin ligase for p27 (Kip1) . Molec. Cell. Biol. 29: 547-558, 2009. Note: Erratum: Molec. Cell. Biol. 29: 3241 only, 2009.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

149 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Ring finger protein 123

Subcellular Location

Cytoplasm.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.