Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband®

About this product

Boster Bio Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband® catalog # A02427-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02427-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sodium-hydrogen antiporter 3 regulator 1 is a regulator of Sodium-hydrogen antiporter 3. It is encoded by the gene SLC9A3R1. It is mapped to 17q25.1. This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.

Synonyms

Na (+) /H (+) exchange regulatory cofactor NHE-RF1; NHERF-1; Ezrin-radixin-moesin-binding phosphoprotein 50; EBP50; Regulatory cofactor of Na (+) /H (+) exchanger; Sodium-hydrogen exchanger regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; SLC9A3R1; NHERF; NHERF1

Gene Name

SLC9A3R1

Gene ID

9368

UniProt

O14745

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human EBP50/NHERF-1/SLC9A3R1 recombinant protein (Position: K69-R338) .

Clonality

Polyclonal

Tissue Specificity

Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.

Applications

ELISA, IHC, WB

Field of Research

Actin Binding Proteins, Actin, etc., Adapters, Cancer, Cell Type Markers, Cytoskeleton, Cytoskeleton/ECM, Extracellular Matrix, Invasion/Microenvironment, Microfilaments, Signal Transduction, Tags & Cell Markers, Transmembrane

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli. Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.

References & Citations

1. Helms, C., Cao, L., Krueger, J. G., Wijsman, E. M., Chamian, F., Gordon, D., Heffernan, M., Daw, J. A. W., Robarge, J., Ott, J., Kwok, P.-Y., Menter, A., Bowcock, A. M. A putative RUNX1 binding site variant between SLC9A3R1 and NAT9 is associated with susceptibility to psoriasis. Nature Genet. 35: 349-356, 2003. 2. Karim, Z., Gerard, B., Bakouh, N., Alili, R., Leroy, C., Beck, L., Silve, C., Planelles, F., Urena-Torres, P., Grandchamp, B., Friedlander, G., Prie, D. NHERF1 mutations and responsiveness of renal parathyroid hormone. New Eng. J. Med. 359: 1128-1135, 2008.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

38779 MW

Observed Molecular Weight

50 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

SLC9A3 regulator 1

Subcellular Location

Apical cell membrane. Cytoplasm. Endomembrane system. Peripheral membrane protein. Filopodium. Ruffle. Microvillus.

Protein Name

BAG family molecular chaperone regulator 1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.