Anti-CDC42 Antibody Picoband®

About this product

Boster Bio Anti-CDC42 Antibody Picoband® catalog # A00119. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00119

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene.CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.

Synonyms

Cell division control protein 42 homolog; G25K GTP-binding protein; CDC42

Gene Name

CDC42

Gene ID

998

UniProt

P60953

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CDC42 recombinant protein (Position: K16-C188) .

Clonality

Polyclonal

Tissue Specificity

Widely expressed. .

Applications

ELISA, Flow Cytometry, IHC, WB

Field of Research

Associated Proteins, Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Differentiation, Epigenetics and Nuclear Signaling, G Protein Signaling, Intracellular, Nucleotide Messenger, Proto-Oncogenes, Ras Family, Second Messenger, Signal Transduction, Signaling Pathway, Small G Proteins, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation. Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.

References & Citations

1. Shinjo K, Koland JG, Hart MJ, Narasimhan V, Johnson DI, Evans T, Cerione RA (December 1990) . 2. Munemitsu S, Innis MA, Clark R, McCormick F, Ullrich A, Polakis P (November 1990) .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21298 MW

Observed Molecular Weight

21 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cell division cycle 42 (GTP binding protein, 25kDa)

Subcellular Location

Centrosome. Spindle. Cell membrane. Lipid-anchor. Cytoplasmic side. Midbody.

Protein Name

GTPase Hras

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.