Anti-MECP2 Antibody Picoband®

About this product

Boster Bio Anti-MECP2 Antibody Picoband® catalog # A00047. Tested in ELISA, Flow Cytometry, IF, IHC, ICC/IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00047

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MECP2 (methyl CpG binding protein 2) is a gene that encodes the protein MECP2. It is mapped to Xq28. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD) . Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms

Methyl-CpG-binding protein 2; MeCp-2 protein; MeCp2; MECP2

Gene Name

MECP2

Gene ID

4204

UniProt

P51608

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MECP2 recombinant protein (Position: K119-R453) .

Clonality

Polyclonal

Tissue Specificity

Present in all adult somatic tissues tested.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC/IF, WB

Field of Research

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) -containing DNA, with a preference for 5-methylcytosine (5mC) .

References & Citations

1. Amir RE, Van den Veyver IB, Wan M, Tran CQ, Francke U, Zoghbi HY (October 1999) . Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat. Genet. 23 (2) : 185–8. 2. Chahrour M, et al. (2008) . MECP2, a key contributor to neurological disease, activates and represses transcription. Science. 320 (5880) : 1224–9.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

52 kDa

Observed Molecular Weight

75 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Methyl-CpG binding protein 2

Subcellular Location

Nucleus.

Protein Name

Vascular endothelial growth factor A

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.