Anti-OLFM4 Antibody Picoband®

About this product

Boster Bio Anti-OLFM4 Antibody Picoband® catalog # A04094-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04094-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Olfactomedin 4 is a protein that in humans is encoded by the OLFM4 gene. This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion.

Synonyms

Olfactomedin-4; OLM4; Antiapoptotic protein GW112; G-CSF-stimulated clone 1 protein; hGC-1; hOLfD; OLFM4; GW112; UNQ362; PRO698

Gene Name

OLFM4

Gene ID

10562

UniProt

Q6UX06

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human OLFM4 recombinant protein (Position: D91-Q510) .

Clonality

Polyclonal

Tissue Specificity

Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level) . Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues.

Applications

ELISA, IHC, WB

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.

References & Citations

1. Dassen H, Punyadeera C, Delvoux B, Schulkens I, Marchetti C, Kamps R, Klomp J, Dijcks F, de Goeij A, D'Hooghe T, Kyama C, Ederveen A, Dunselman G, Groothuis P, Romano A (2010) . Olfactomedin-4 regulation by estrogen in the human endometrium requires epidermal growth factor signaling. Am. J. Pathol. 177 (5) : 2495–508. 2. van der Flier LG, Haegebarth A, Stange DE, van de Wetering M, Clevers H (2009) . OLFM4 is a robust marker for stem cells in human intestine and marks a subset of colorectal cancer cells.Gastroenterology. 137 (1) : 15–7.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

211344 MW

Observed Molecular Weight

75 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Olfactomedin 4

Subcellular Location

Extracellular space. Mitochondrion.

Protein Name

Chromodomain-helicase-DNA-binding protein 2

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.