Anti-BHC80/PHF21A Antibody Picoband®

About this product

Boster Bio Anti-BHC80/PHF21A Antibody catalog # A08507-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A08507-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PHD finger protein 21A is a protein that in humans is encoded by the PHF21A gene. The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535) /histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) .

Synonyms

PHD finger protein 21A; BHC80a; BRAF35-HDAC complex protein BHC80; PHF21A; BHC80; KIAA1696; BM-006

Gene Name

PHF21A

Gene ID

51317

UniProt

Q96BD5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human BHC80/PHF21A recombinant protein (Position: M1-Q97) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in brain. Expressed at much lower level in other tissues.

Applications

ELISA, Flow Cytometry, IF, IHC, WB

Field of Research

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of 'Lys-4' of histone H3 in vitro, suggesting a role in demethylation regulation.

References & Citations

1. Nagase T, Kikuno R, Hattori A, Kondo Y, Okumura K, Ohara O (2000) . Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7 (6) : 347–55. 2. Iwase S, Januma A, Miyamoto K, Shono N, Honda A, Yanagisawa J, Baba T (Sep 2004) . Characterization of BHC80 in BRAF-HDAC complex, involved in neuron-specific gene repression. Biochem. Biophys. Res. Commun. 322 (2) : 601–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

75-95 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

PHD finger protein 21A

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.